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More parameters, more informative phenotypes: Systematic phenotyping approaches
Recent years have witnessed the growth of functional genomics projects focusing on the use of the laboratory mouse as a model of human disease. The single gene approach has past, and we are now entering a new phase where scientists will think in terms of networks and pathways. The Centre for ImmunoPHEnomics – CIPHE is a new institute dedicated to phenogenomic studies. With its cutting-edge expertise in mouse genetics and immunology, CIPHE aims to develop and analyze, in a massively parallel and standardized mode, mouse KO/KI models, to understand the function of the mouse immune system under normal and infectious conditions. CIPHE has the unique ability to phenotype all the cellular components of the immune system, under normal conditions or confronted with pathogens by, integrating current cutting-edge research and technical knowledge in cytometry.
To achieve this goal and to be able to characterize multiple samples from large cohorts of animals in a single day of experiments, we have been using the Invitrogen Attune NxT Flow Cytometers (most recently the Attune NxT V6 configuration) coupled to an Autosamplers. In this presentation, I will discuss how this instrument is central to many assays developed at CIPHE and how it gave us a competitive and strategical advantage in flow and mass cytometry as well as single cell genomic pipelines. Results of a full characterization of instrument performances, as well as a strategy for rational design of multicolor panels on the Attune NxT instrument, will also be presented.
Three goals/objectives the webinar will cover:
- The importance of instrument standardization to ease protocol transfers and help streamline multicolor panel design.
- List the rational steps to characterize instrument performance for high content applications (multicolor panel design).
- Demonstrate the flexibility/strategical importance of the Attune NxT Flow Cytometer in a busy flow cytometry core laboratory.
Click here to watch.